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Quantitation of Intra-peritoneal Ovarian Cancer Metastasis
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JoVE Journal Medicina
Quantitation of Intra-peritoneal Ovarian Cancer Metastasis

Quantitation of Intra-peritoneal Ovarian Cancer Metastasis

DOI:
10.3791/53316-v

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10:58 min

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July 18, 2016

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, , ,
1Department of Chemistry & Biochemistry, Harper Cancer Research Institute,University of Notre Dame

Capítulos

  • 00:05Título
  • 01:06In vivo Imaging of ID8 Murine Ovarian Cancer Cells
  • 02:19Mouse Dissection and Image Acquisition
  • 05:04Quantification of Tumor Burden in the Flourescence Images
  • 09:12Results: Quantitation of Ovarian Cancer Metastasis
  • 10:19Conclusion

Summary

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Tadução automática

Ovarian cancer metastasis is characterized by numerous diffuse intra-peritoneal lesions, such that accurate visual quantitation of tumor burden is challenging. Herein we describe a method for in situ and ex vivo quantitation of metastatic tumor burden using red fluorescent protein (RFP)-labeled tumor cells and optical imaging.

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Keywords: Intra-peritoneal Ovarian Cancer MetastasisSyngeneic Orthotopic Xenograft Murine ModelFluorescent SpectraRelative Tumor BurdenMulti-spectral AcquisitionReflectance ImagePeritoneal CavityLiverOmentumPancreasStomachDiaphragmSmall And Large Intestines

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