É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:04Micropost Array Cell Culture Preparation
- 02:43Fixing & Staining Cells
- 03:48Imaging Cells on the Micropost Array
- 05:19Image Analysis with Open Source Software “MechProfiler”
- 08:04Results: Mechano-profiling of Bone Cancer Cell Lines
- 09:38Conclusion
Described are protocols for quantifying mechanical interactions between adherent cells and microstructured substrates. These interactions are closely linked to essential cell behaviors including migration, proliferation, differentiation, and apoptosis. The protocols present an open-source image analysis software called MechProfiler, which enables determination of involved forces for each micropost.
