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人 Intraepidermal 神经纤维内线粒体三维成像及分析

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

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人 Intraepidermal 神经纤维内线粒体三维成像及分析

DOI:

10.3791/53369-v

•

10:31 min

•

September 29, 2017

•

Hussein S. Hamid, John M. Hayes, Eva L. Feldman, Stephen I. Lentz

¹University of Michigan Medical School, ²Department of Neurology,University of Michigan, ³Department of Internal Medicine,University of Michigan

Capítulos

00:05Título
01:02Fluorescence Immunohistochemistry: Day 1
02:27Fluorescence Immunohistochemistry: Day 2
03:21Fluorescence Immunohistochemistry: Day 3
04:22Confocal Imaging
06:00Image Processing
07:03Isolation of Nerve-specific Mitochondrial Signal
08:41Results: Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
09:39Conclusion

Summary

Tadução automática

该协议使用三维 (3D) 成像和分析技术来可视化和量化神经特异性线粒体。这些技术适用于在其他情况下, 一个荧光信号被用来隔离一个子集的数据, 从另一个荧光信号。

Tags

Keywords: 3D Imaging Mitochondria Intraepidermal Nerve Fibers Fluorescence Immunohistochemistry PGP9.5 PDH Secondary Antibodies Signal Enhancement Epidermal Nerve Fibers Neurological Complications

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