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Biology

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JoVE Journal Biology
Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
 

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood

Article DOI: 10.3791/53384-v • 11:00 min • December 23rd, 2015
December 23rd, 2015 •
1, 2, 1, 1, 1, 1, 1
1Department of Medicine, University of Cambridge, 2Papworth Hospital

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This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.

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Keywords: Blood Outgrowth Endothelial Cells BOECs Endothelial Progenitors Endothelial Dysfunction Vascular Disorders Primary Endothelial Cells Cell Culture Patient-derived Endothelial Cell Transplantation Induced Pluripotent Stem Cells IPSCs Nuclear Reprogramming Peripheral Blood
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