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Immunology and Infection

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JoVE Journal Immunology and Infection
Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging
 

Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

Article DOI: 10.3791/53392-v • 07:25 min • April 29th, 2016
April 29th, 2016 •
1,2, 3,4, 1,2,5
1Temasek Life Sciences Laboratory, 2Department of Biological Science, National University of Singapore, 3Bioimaging and Biocomputing Facility, Temasek Life Sciences Laboratory, 4Histology and Light Microscopy Core, Gladstone Institutes, 5Pacific Biosciences Research Center, University of Hawai'i at Mānoa

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The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.

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Physiological Responses Drosophila Sensory Neurons Lipid Pheromones Live Calcium Imaging Gustatory Receptors Sensory Neurobiology Intact Live Animal Anesthetizing Cover Slip Clear Nail Polish Dissecting Microscope Foreleg Tarsal Segments Tape Proboscis Labellum PAP Pen Anesthesia Wear Off Measurements Stock Ligand Solution PBST
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