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Прикосновение Универсальный мышиной модели подкорковых белого вещества для изучения дегенерации аксонов и белого вещества нейробиологии
JoVE Journal
Medicina
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JoVE Journal Medicina
A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology
DOI:

08:36 min

March 17, 2016

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Capítulos

  • 00:05Título
  • 01:03White Matter Stroke Induction: Medial Angled Approach
  • 05:12Tissue Processing for Protein or RNA Analysis
  • 06:47Results: Focal White Matter Stroke Using Both Medial and Posterior Angled Approach
  • 08:02Conclusion

Summary

Tadução automática

Here we present methodology for the production of a focal stroke in murine white matter by local injection of an irreversible endothelial nitric oxide synthase (eNOS) inhibitor (L-Nio). Presented are two stereotactic variations, retrograde neuronal tracing, and fresh tissue labeling and dissection that expand the potential applications of this technique.

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