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Capítulos
- 00:05Título
- 00:48Protocol for Interphasic Xenopus Laevis Egg Extract
- 05:38Protocol for In Vitro Reconstitution of Chromatin Decondensation
- 08:56Results: Chromatin Decondensation is ATP and GTP Dependent
- 09:48Conclusion
The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.
