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Immunology and Infection

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JoVE Journal Immunology and Infection
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
 

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Article DOI: 10.3791/53408-v • 12:16 min • December 17th, 2015
December 17th, 2015 •
1, 1,2,3
1Philips Institute for Oral Health Research, Virginia Commonwealth University, 2Department of Microbiology and Immunology, Virginia Commonwealth University, 3Department of Biochemistry, Virginia Commonwealth University

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This article presents two protocols: one to measure anaerobic bacteria that can successfully invade and survive within the host, and the other to visualize anaerobic bacteria interacting with host cells. This study can be applied to any cultivable anaerobe and any eukaryotic cell type.

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Keywords: Porphyromonas Gingivalis Anaerobic Bacteria Host Cell Invasion Antibiotic Protection Assay Fluorescent Microscopy BCECF-AM TRITC Phalloidin DAPI Z-stack Imaging
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