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Capítulos
- 00:05Título
- 00:35Cotyledon specimen Sky Drop Mounting
- 02:35Variable-angle Epifluorescence Microscopy (VAEM) Observation and Movie Acquisition
- 03:42GFP-labeled Dot Residence Time Quantification by Kymograph Analysis
- 05:19Results: Representative GFP-PATROL1 Dot Residence Time Analysis by Kymography
- 06:02Conclusion
Het doel van dit protocol is om te demonstreren hoe fluorescent-gelabelde eiwit dynamiek op plantencel oppervlakken met een variabele hoek epifluorescentiemicroscopie, waaruit blijkt knipperende stippen van GFP-gelabeld PATROL1, een membraan mensenhandel eiwit te controleren, in de cel cortex van de stomatale complex in Arabidopsis thaliana.
