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Light-driven Enzymatic Decarboxylation
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Química
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JoVE Journal Química
Light-driven Enzymatic Decarboxylation

Light-driven Enzymatic Decarboxylation

DOI:
10.3791/53439-v

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09:58 min

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May 22, 2016

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, , , ,
1Faculty of Biology and Biotechnology,Ruhr Universität Bochum, 2Biology and Biotechnology,Delft University of Technology

Capítulos

  • 00:05Título
  • 01:00Production of Recombinant Decarboxylase in Escherichia coli
  • 03:42Purification of OleTJE
  • 05:22Biotransformation of Fatty Acids Using OleT
  • 06:50Analysis of Biotransformation Products
  • 08:26Results: Analysis of the Light-catalyzed Biotransformations by Gas Chromatography
  • 09:19Conclusion

Summary

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Tadução automática

We describe a protocol for the light-catalyzed generation of hydrogen peroxide — a cofactor for oxidative transformations.

Tags

Enzymatic DecarboxylationLight-driven BiocatalysisOleT EnzymeFatty Acid DecarboxylationTerminal Alkyne SynthesisHydrogen Peroxide GenerationE. Coli ExpressionProtein PurificationCell LysisCell-free Extract

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