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Эффективное млекопитающих выражение клеток и Пошаговый Очистка внеклеточной гликопротеинов для кристаллизации
JoVE Journal
Biologia
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JoVE Journal Biologia
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
DOI:

07:08 min

December 23, 2015

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Capítulos

  • 00:05Título
  • 01:02Large-scale Culture and Transient Transfection of 293F Cells
  • 02:40Purification
  • 04:58Results: Expression and Purification of Secreted Mammalian Proteins
  • 06:29Conclusion

Summary

Tadução automática

This is a quick, cost-efficient protocol for the production of secreted, glycosylated mammalian proteins and subsequent single-step purification with sufficient yields of homogenous protein for X-ray crystallography and other biophysical studies.

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