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Ställa in en parallell segmenterat flöde Kolumn och Aktivera Multiplexed Detection
JoVE Journal
Química
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JoVE Journal Química
Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
DOI:

08:01 min

December 15, 2015

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Capítulos

  • 00:05Título
  • 01:15Setup of High Performance Liquid Chromatography (HPLC) Instrument and UV-Vis/Mass Spectrometry (MS) Detectors
  • 02:51Preparation of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Radical Reagent and System Detector
  • 04:00Setup of Parallel Segmented Flow (PSF) Column
  • 05:00Tuning the PSF Column for Multiplexed Detection
  • 06:18Results: Multiplexed Detection of a Coffee Sample
  • 07:11Conclusion

Summary

Tadução automática

Here, we present a protocol for the operation and tuning of parallel segmented flow chromatography columns to enable multiplexed detection.

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