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Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

DOI:
10.3791/53453-v

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12:12 min

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February 08, 2016

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, , , ,
1Department of Pediatrics,Emory University School of Medicine, 2Department of Biochemistry,Emory University School of Medicine

Capítulos

  • 00:05Título
  • 01:00Generate Survival Curves to Determine Needed Doses of UV Irradiation
  • 03:01Screening for Mutagenized Auxotrophic Yeast Strains and Yeast Transformation
  • 05:42Oral Gavage of Mice with Transformed Yeast
  • 07:50Harvest of Murine Peyer’s Patches and Isolation of Viable Yeast Colonies
  • 10:03Representative Results
  • 11:28Conclusion

Summary

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Presented here is a unified description of techniques that can be used to develop, transform, administer, and test heterologous protein expression of the probiotic yeast Saccharomyces boulardii.

Tags

Keywords: Probiotic YeastOral GavageUV Irradiation5-FOAUra3 MutantOxytrophic StrainsGenetic ManipulationOral VaccinationGastrointestinal Therapeutics

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