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Capítulos
- 00:05Título
- 00:38Measuring Buffering Power with an Extracellular Flux Instrument
- 02:30Measuring Buffering Power with an External pH Meter
- 03:43Extracellular Flux Assay
- 04:48Results: Contributions of Glycolysis and Respiration to the Proton Production Rate (PRR) after Correction
- 05:49Conclusion
Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.
