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Preparazione di Thermoresponsive Nanostrutturati Superfici Tissue Engineering
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
DOI:

12:22 min

March 01, 2016

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Capítulos

  • 00:05Título
  • 01:15Synthesis of Polystyrene-block-poly(N-isopropylacrylamide) by Two-step Reversible Addition-fragmentation Chain Transfer (RAFT) Radical Polymerization
  • 02:42Preparation of Silanized Hydrophobic Modified Glass Substrates
  • 03:49Preparation of Langmuir Films and Film-transferred Surface
  • 06:59Culturing Cells and Optimizing Cell Adhesion and Detachment on the Langmuir Film Transferred Surface
  • 09:32Results: Fabrication of Langmuir Film-transferred Surface and Evaluation of Cell Adhesion, Detachment, and Cell Sheet Recovery
  • 11:20Conclusion

Summary

Tadução automática

Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.

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