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Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools
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Biologia
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JoVE Journal Biologia
Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

DOI:
10.3791/53469-v

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10:41 min

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December 16, 2015

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, , , ,
1Buchmann Institute for Molecular Life Sciences and Institute of Biochemistry II, School of Medicine,Goethe University

Capítulos

  • 00:05Título
  • 00:523D Cell Shape Model Reconstruction with IMOD
  • 03:08Fluorescently-labeled Structure Tracking with Endrov
  • 05:33Cortical Actomyosin Flow Analysis with PIVIab
  • 08:04Results: Representative C. elegans Developmental Tracking Analyses
  • 09:39Conclusion

Summary

Tadução automática

Tadução automática

Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.

Tags

Keywords: C. ElegansDevelopmental ProcessesTime-lapse MicroscopyTrackingQuantificationOpen-source ToolsIMODEndrovPIVlabCell TrackingLineage TracingVesicle Flow

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