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Genom-dækkende Kortlægning af lægemiddel-DNA interaktioner i celler med COSMIC (Tværbinding af små molekyler til at isolere Chromatin)
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Biologia
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JoVE Journal Biologia
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)

Genom-dækkende Kortlægning af lægemiddel-DNA interaktioner i celler med COSMIC (Tværbinding af små molekyler til at isolere Chromatin)

DOI:
10.3791/53510-v

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10:05 min

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January 20, 2016

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, , , , ,
1Department of Biochemistry,University of Wisconsin–Madison, 2Department of Electrical and Computer Engineering,University of Wisconsin–Madison, 3Graduate Program in Cellular and Molecular Biology,University of Wisconsin–Madison, 4The Genome Center,University of Wisconsin–Madison

Capítulos

  • 00:05Título
  • 00:48Crosslinking in Live Cells
  • 03:19Isolation of Chromatin
  • 05:14Capture of Ligand-DNA Crosslinks and Isolation of Affinity-purified DNA
  • 07:52Results: COSMIC Indentifies Genome-wide Binding Events of Polyamides
  • 09:21Conclusion

Summary

Tadução automática

Tadução automática

Identificere de direkte mål for genom-targeting molekyler stadig en stor udfordring. For at forstå hvordan DNA-bindende molekyler engagere genomet, vi udviklet en metode, der bygger på krydsbinding af små molekyler til at isolere kromatin (COSMIC).

Tags

Genome-wide MappingDrug-DNA InteractionsCOSMICCrosslinkingSmall MoleculesChromatinPolyamidesDNA-targetingMechanism Of ActionTherapeutic AgentsDNA-targeting MoleculesPluripotent Stem CellsPhoto-cross-linkingUV RadiationCell DissociationEnzymeE8 Media

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