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A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
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Biologia
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JoVE Journal Biologia
A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

DOI:
10.3791/53513-v

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12:18 min

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April 17, 2016

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, , , , ,
1Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center,Gyeongsang National University, 2Biotechnology Research Institute,Chinese Academy of Agricultural Sciences

Capítulos

  • 00:05Título
  • 01:12Screening for Altered Phototropic or Gravitropic Responses
  • 04:09Analysis of Seedling Bending Angles
  • 05:32Hypocotyl Assay: HPTS Dye-loading
  • 07:30Callose Aniline Blue Staining
  • 08:44Analysis of Callose Signal
  • 09:45Results: Screening AtGSL8 RNAi Plants
  • 11:06Conclusion

Summary

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This article describes the methods for screening the genes controlling plasmodesmal permeability and hence auxin gradient during tropic response. This includes the measurement of the degree of tropic response in hypocotyl of Arabidopsis thaliana and checking plasmodesmal permeability by 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) loading and finally callose level assessment.

Tags

ArabidopsisEtiolated HypocotylsPlasmodesmal GatingCalloseTropic ResponseCell-to-cell SignalingGSL8Glucose SynthaseMurashige And Skoog MediumPhototropic ResponseGravitropic Response

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