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从鼠耳和尾组织生成主成纤维细胞培养

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

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从鼠耳和尾组织生成主成纤维细胞培养

DOI:

10.3791/53565-v

•

06:35 min

•

January 10, 2016

•

Muznah Khan, Stephan Gasser²

¹Immunology Program, Department of Microbiology,Yong Loo Lin School of Medicine, National University of Singapore, ²NUS Graduate School for Integrative Sciences and Engineering,National University of Singapore

Capítulos

00:05Título
00:42Extraction of Fibroblasts from Ear and Tail Tissues
03:20Cell Culture and Sub-Culturing of Fibroblasts
05:05Results: Fibroblast Culture Hardiness and Purity
06:03Conclusion

Summary

Tadução automática

我们描述用于产生从耳朵和小鼠的尾部的成纤维细胞初级一个简单而快速的实验程序。该过程不需要特殊的动物训练和可用于成纤维细胞培养的产生从存储在RT至10天耳朵。

Tags

Primary Fibroblast Mouse Ear Mouse Tail Cell Isolation Collagenase Digestion Cell Strainer Cell Suspension Cell Washing

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