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减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默

JoVE Journal
Biologia

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JoVE Journal Biologia

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

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减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默

DOI:

10.3791/53586-v

•

09:16 min

•

October 11, 2015

•

Claudia Baumann, Maria M. Viveiros

¹Department of Physiology and Pharmacology,College of Veterinary Medicine, University of Georgia

Capítulos

00:05Título
00:54Collecting Mouse Oocytes
02:08Oocyte Microinjection
04:42Culturing and Staining the Injected Oocytes
07:52Results: Disruption of Pericentrin Expression in Oocytes during Meiotic Division
08:37Conclusion

Summary

Tadução automática

在这里，我们提出了一个协议，用于特定的siRNA介导的mRNA枯竭其次是免疫荧光分析来评估减数分裂纺锤体组装和组织在小鼠卵母细胞。这个协议是适合体外转录耗尽的和不同的主轴和/或微管组织中心相关因子中的卵母细胞的功能评价。

Tags

Keywords: Meiotic Spindle SiRNA Chromosome Segregation Aneuploidy Microtubule Spindle Apparatus MTOC Mouse Oocytes Immunofluorescence Analysis

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