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双工数字PCR方法的同时定量<em>肠球菌。</em>和人的粪便相关HF183标记的水域
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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
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双工数字PCR方法的同时定量<em>肠球菌。</em>和人的粪便相关HF183标记的水域

DOI:
10.3791/53611-v

•

12:14 min

•

March 09, 2016

•

, ,
1Department of Microbiology,Southern California Costal Water Research Project Authority

Capítulos

  • 00:05Título
  • 00:56Assay Mixture Preparation
  • 02:24Droplet Generation and PCR Plate Setup
  • 05:17Thermal Cycling and Droplet Reading
  • 08:01Data Analysis and Reporting
  • 09:46Results: Duplex Digital PCR is a Robust Assay for Simultaneous Quantification of General and Human-associated Fecal Contamination
  • 11:18Conclusion

Summary

Tadução automática

Tadução automática

该原稿描述了一种双工数字PCR分析可以用于同时定量肠球菌和HF183遗传标记在娱乐用水一般与人类相关的粪便污染的指标。

Tags

Duplex Digital PCR AssayEnterococcus Spp.HF183 MarkerFecal ContaminationWater Quality MonitoringQPCRMaster MixDroplet GenerationPositive ControlsNo Template Controls (NTCs)

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