Journal
/
Biologia do Desenvolvimento
/
Primær endodermal epithelcellekultur fra blommesækken membran af japanske vagtelembryoner
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Primær endodermal epithelcellekultur fra blommesækken membran af japanske vagtelembryoner

DOI:
10.3791/53624-v

•

11:53 min

•

March 10, 2016

•

, , ,
1Department of Animal Science and Technology,National Taiwan University

Capítulos

  • 00:05Título
  • 01:03Preparation of Basal Medium and Wash Solutions
  • 01:57Collection of Primary Endodermal Epithelium from Yolk Sac Membranes
  • 05:06Digestion of Endoderm by Collagenase
  • 08:26Results: Culture System Performance and its Application to Study Lipid Utilization
  • 10:30Conclusion

Summary

Tadução automática

Tadução automática

To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.

Tags

Avian Endodermal Epithelial CellsYolk Sac MembraneJapanese Quail EmbryosEx Vivo Explant ProcedureNutrient UtilizationLipid UtilizationYolk LipidsAvian EmbryogenesisFunctional Endodermal Epithelial CellsAvian Embryonic Development10x PBS SolutionGrowth MediumEECsDMEM/F-12Newborn Calf SerumPenicillin Streptomycin Amphotericin SolutionPH MeterHCl AdjustmentFertilized Quail Eggs

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/53624?article_type=v

Effektiv Gene Transfer i Chick nethinder for primær cellekultur Studies: An<em> Ex-ovo</em> Elektroporation Approach
check_url/pt/53624?article_type=v

Dyrk Primær Nasal epitelceller fra Børn og Omprogrammering ind induceret pluripotente stamceller
check_url/pt/53624?article_type=v

Isolering og karakterisering af enkeltceller fra zebrafisk embryoer
check_url/pt/53624?article_type=v

Storstilet produktion af cardiomyocytter fra menneskelige pluripotente stamceller Ved hjælp af en meget reproducerbar lille molekyle-Based Differentiering Protocol
check_url/pt/53624?article_type=v

Affinity Labeling Påvisning af endogene receptorer fra zebrafisk embryoer
check_url/pt/53624?article_type=v

Biosensing Motor Neuron Membran Potential i Live Zebrafish Embryoer
check_url/pt/53624?article_type=v

Hele-celle patch-clamp optagelser af isolerede primære epitelceller fra epididymis
check_url/pt/53624?article_type=v

Afledning af stamcellelinjer fra mus præimplantations embryoner
check_url/pt/53624?article_type=v

AFM og Microrheology i cellen zebrafisk Embryo æggeblomme
check_url/pt/53624?article_type=v

Anvendelse af Aorta-gonade-mesonephros eksplantat kultur System i udviklingsmæssige bloddannelsen

Read Article