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बड़े पैमाने पर स्कैनिंग संचरण इलेक्ट्रॉन माइक्रोस्कोपी (Nanotomy) स्वस्थ और घायल Zebrafish मस्तिष्क की
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain
DOI:

10:09 min

May 25, 2016

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Capítulos

  • 00:05Título
  • 01:10Larvae Fixation and Embedding
  • 02:38Sectioning, Contrasting, and Mounting
  • 04:28Mounting the Sample in the Scanning Electron Microscope and Acquiring Images
  • 06:40Data Analysis
  • 07:33Results: Large Scale Scanning Transmission Electron Microscopy of Zebrafish Brain
  • 08:48Conclusion

Summary

Tadução automática

Large-scale 2D electron microscopy (EM), or nanotomy, is the tissue-wide application of nanoscale resolution EM. Here we describe a universal method for nanotomy applied to investigate the zebrafish larval brain in health and upon non-invasive brain injury.

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