/
/
Isolamento di leucociti infiltranti da mouse pelle utilizzando enzimatica Digest e Separazione Gradient
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:58Flank Skin Harvest and Infiltrating Leukocyte Isolation
- 04:28Results: Oxazolone (Ox) Challenge Results in Pronounced T Cell and Neutrophil Flank Skin Infiltration
- 06:17Conclusion
This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.
Tags
IsolationInfiltrating LeukocytesMouse SkinEnzymatic DigestGradient SeparationCellular MechanismsMolecular MechanismsMouse ModelsSkin PathologiesAtopic DermatitisResident Cell PopulationsSkin HarvestingVisual DemonstrationPrecisionPracticeSurgical ScissorsFlank SkinND4 Swiss-Webster MouseSurgical Blade50 Milliliter Conical TubeHBSSEDTAHEPESFBS Incubation
