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Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
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Bioengenharia
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JoVE Journal Bioengenharia
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

DOI:
10.3791/53659-v

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09:21 min

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January 04, 2016

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, ,
1Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology,Academic Medical Center, 2Laboratory of Chemical Biology,Institute of Complex Molecular Systems (ICMS), 3Department of Biomedical Engineering,Eindhoven University of Technology

Capítulos

  • 00:05Título
  • 00:55The Bile Acid Sensor
  • 02:12FRET Measurement Using Confocal Microscopy
  • 05:39Image Analysis
  • 07:42Results: Confocal-based FRET Experiment with BAS
  • 08:37Conclusion

Summary

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Tadução automática

We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.

Tags

Genetically Encoded FRET-based Bile Acid SensorReal-time MonitoringIntracellular Bile Acid DynamicsSpacial ResolutionTemporal ResolutionMetabolic QuestionsGlucose MetabolismLipid MetabolismEnergy MetabolismSingle Living CellsLabeling Bile AcidsCell Transport And DynamicsProtein LocalizationFXR Nuclear Bile Acid ReceptorLigand Binding DomainCerulean FluorophoreCitrine FluorophoreInter-molecular InteractionsNuclear Localization Signal

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