/
/
Las fibras serotoninérgicos proyección de imagen en la médula espinal de ratón El uso de la claridad / Técnica CÚBICO
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:08Transparent Mouse Spinal Cord Preparation
- 02:30Mouse Spinal Cord Clearance
- 04:59Immunofluorescent Imaging
- 07:18Results: Representative Serotonergic Fiber Imaging
- 08:55Conclusion
Supraspinal projections are important for pain perception and other behaviors, and serotonergic fibers are one of these fiber systems. The present study focused on the application of the combined CLARITY/CUBIC protocol to the mouse spinal cord in order to investigate the termination of these serotonergic fibers.
Tags
CLARITY/CUBIC TechniqueSerotonergic FibersMouse Spinal CordNeuroanatomyTraditional Histology3D FeaturesIn Situ ObservationVectorsCentral Nervous SystemCardiovascular SystemTracing MethodPlastic Dissection StageFume HoodAnesthetized MouseChest OpeningHeart ExposurePeristaltic Pump TubingSaline WashBlood Perfusion
