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Novel Metrics à Caractériser Embryonic Allongement du nématode<em> Caenorhabditis elegans</em
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Novel Metrics to Characterize Embryonic Elongation of the Nematode Caenorhabditis elegans
DOI:

10:46 min

March 28, 2016

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Capítulos

  • 00:05Título
  • 00:52Characterizing Early Elongation Using 4D (Time Lapse Z-stack) DIC Imaging
  • 03:47Characterizing Late Elongation Using Flow Cytometry
  • 08:23Results: 4D-DIC versus Flow Cytometry for Sizing Nematode Larvae
  • 09:32Conclusion

Summary

Tadução automática

Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo.

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