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Geregisseerd Evolution Method in<em> Saccharomyces cerevisiae</em>: Mutant Bibliotheek Creatie en Screening
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Biologia
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JoVE Journal Biologia
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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Geregisseerd Evolution Method in<em> Saccharomyces cerevisiae</em>: Mutant Bibliotheek Creatie en Screening

DOI:
10.3791/53761-v

•

10:50 min

•

April 01, 2016

•

, ,
1Department of Biocatalysis,Institute of Catalysis, CSIC

Capítulos

  • 00:05Título
  • 00:42Mutant Library Construction
  • 05:46High-Throughput Screening Assay
  • 07:54Results: Successful Construction and Screening of an AAO Mutant Library
  • 09:42Conclusion

Summary

Tadução automática

Tadução automática

We present a detailed protocol to construct and screen mutant libraries for directed evolution campaigns in Saccharomyces cerevisiae.

Tags

Directed EvolutionSaccharomyces CerevisiaeMutant LibraryAryl-alcohol Oxidase (AAO)Random MutagenesisPCRIn Vivo SplicingOverlapping RegionsHigh-fidelity PCRScreening

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