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用优化的n-甲基 d-葡乙胺保护恢复法制备急性脑切片

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

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用优化的n-甲基 d-葡乙胺保护恢复法制备急性脑切片

DOI:

10.3791/53825-v

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10:53 min

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February 26, 2018

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Jonathan T. Ting*¹, Brian R. Lee*¹, Peter Chong, Gilberto Soler-Llavina, Charles Cobbs, Christof Koch, Hongkui Zeng, Ed Lein

¹Cell Types Program,Allen Institute for Brain Science, ²The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment,Swedish Neuroscience Institute

Capítulos

00:04Título
01:05Setup of the Slicing Station
03:15Brain Dissection and Slicing
06:32Optimized NMDG Protective Recovery Procedure
08:05Results: Improved Neuronal Preservation and Reliability of Gigaohm Seal Formation with the Optimized NMDG Protective Recovery Method
09:38Conclusion

Summary

Tadução automática

该协议演示了一种优化的N-甲基 D-葡乙胺 (NMDG) 脑切片制备保护恢复方法的实现。一个单一的媒体配方是用来可靠地获得健康的脑切片从动物的任何年龄和不同的实验应用。

Tags

Keywords: Brain Slices NMDG Protective Recovery Method Patch Clamp Electrophysiology Neuronal Preservation Adult Mouse Brain NMDG HEPES ACSF Brain Slice Recovery Chamber HEPES ACSF Agarose Embedding

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