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Kombine bir 3D doku mühendisliği<em> İn Vitro</em> /<em> Siliko</emSpesifik mutasyonel Arka İlaç Etkinliği tahmin için> Akciğer Tümör Modeli
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds
DOI:

13:34 min

April 06, 2016

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Capítulos

  • 00:05Título
  • 01:00Dynamic Culture Conditions and Static Tumor Model Gefitinib Treatment
  • 02:35ELISA Samples Collection and Proliferation Index Determination
  • 05:21In Silico Tumor Model Generation and Anti-EGFR Therapy Simulation in HCC827 Cells
  • 07:13In Silico Simulation Using SQUAD
  • 10:32Results: The Effects of Gefitinib Treatment on Cell Growth in Static and Dynamic Tumor Model Systems
  • 12:42Conclusion

Summary

Tadução automática

We present a three-dimensional (3D) lung cancer model based on a biological collagen scaffold to study sensitivity towards non-small-cell-lung-cancer-(NSCLC)-targeted therapies. We demonstrate different read-out techniques to determine the proliferation index, apoptosis and epithelial-mesenchymal transition (EMT) status. Collected data are integrated into an in silico model for prediction of drug sensitivity.

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