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脂肪库专用SCA1的免疫磁性分离<sup>高</sup>脂肪干细胞(ASCs)
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)
DOI:

08:52 min

August 11, 2016

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Capítulos

  • 00:05Título
  • 00:45Subcutaneous (SQ) and Visceral (VIS) Fat Pad Isolation
  • 02:35Fat Pad Collagenase Digestion
  • 04:20Magnetic Cell Separation
  • 06:31Results: Fat Depot-dependent Expression of Collagenolytic Matrix Metalloproteinases (MMPs)
  • 07:52Conclusion

Summary

Tadução automática

We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.

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