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Uyanık Farelerde Nöroaktif Maddelerin Microiontophoretic Uygulaması ile birlikte Nöronal Faaliyet dışı Kaydı
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
DOI:

11:19 min

May 21, 2016

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Capítulos

  • 00:05Título
  • 00:54Tungsten Electrodes
  • 03:03Multibarrel Glass Pipette Manufacturing
  • 04:26Surgery and Headpost Implantation
  • 06:49Electrophysiological Recording and Microiontophoresis
  • 08:26Results: Effect of the Blockade of the GABAA-receptors on the Spectral and Temporal Response Properties as well as the Repetitive and Rare Sounds
  • 09:58Conclusion

Summary

Tadução automática

We present methods for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. This technique allows the detailed analysis of putative local synaptic inputs to the neuron of interest.

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