Journal
/
/
लेजर की मदद से अलग-अलग प्रकार की कोशिकाओं के Transcriptional प्रोफाइलिंग के लिए एक उपकरण के रूप में Microdissection (एलएएम)
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types
DOI:

09:31 min

May 10, 2016

, ,

Capítulos

  • 00:05Título
  • 00:51Tissue Embedding, Thin Sectioning and Mounting for LAM
  • 04:14LAM
  • 07:32Results: Identification of Genes Expressed Only in the Apomictic Egg Cell
  • 08:33Conclusion

Summary

Tadução automática

Here we present a protocol for laser-assisted microdissection of specific plant cell types for transcriptional profiling. While the protocol is suitable for different species and cell types, the focus is on highly inaccessible cells of the female germline important for sexual and apomictic reproduction in the crucifer genus Boechera.

Vídeos Relacionados

Read Article