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Använda ljus Sheet fluorescensmikroskopi till bild Zebrafish Eye utveckling
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
DOI:

13:01 min

April 10, 2016

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Capítulos

  • 00:05Título
  • 01:34Preparing the Microscope
  • 02:42Preparing the Sample
  • 05:46Image Processing
  • 10:33Results: Light Sheet Fluorescent Microscopy (LSFM) Images
  • 11:58Conclusion

Summary

Tadução automática

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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