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A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis
 

A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis

Article DOI: 10.3791/53968-v • 07:33 min • March 15th, 2016
March 15th, 2016 •
1, 1, 1
1Department of Geobiology, Georg-August University of Göttingen

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The goal of this protocol is to provide users with a set of methods for the high-throughput decapsulation of Lymnaea stagnalis embryos and larvae in preparation for whole mount in situ hybridization, and for subsequent pre- and post-hybridization treatments.

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Whole Mount In Situ Hybridization Gastropod Mollusc Lymnaea Stagnalis Spatial Expression Patterns Embryonic And Larval Whole Mounts Evo-Divo Developmental Biology Genes Expression Gene Interactions Punitive Functions Removal Of Embryonic And Larvae Material Developmental Roadmap Pre-hybridization Steps Disposal Syringe Silicone Tubing Microscope Slide Glass Needle Decapsulated Material Egg Strains
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