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方法在成纤维细胞迁移的规律研究大分子复合物含MRP4，
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
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方法在成纤维细胞迁移的规律研究大分子复合物含MRP4，

DOI:
10.3791/53973-v

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10:43 min

•

May 19, 2016

•

, ,
1Division of Pulmonary Medicine, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 2Department of Physiology,University of Tennessee Health Science Center

Capítulos

  • 00:05Título
  • 01:20Preparation of Cells and Creating Wounds
  • 02:50Monitor Cell Migration and Data Analysis
  • 04:09Förster Resonance Enegry Transfer
  • 07:17Data Analysis
  • 08:34Results: MRP4 Regulation of Fibroblast Migration
  • 09:40Conclusion

Summary

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Tadução automática

MRP4调节各种循环依赖于核苷酸信号事件，包括细胞迁移最近阐明的作用。我们描述一个直接的，而是多方面的方法解开导致该起主要作用的成纤维细胞迁移的微调调控独特MRP4相互作用组鉴定MRP4下游分子的目标。

Tags

MRP4Fibroblast MigrationCyclic AMPFRET ImagingHigh-content MicroscopyCell MigrationProtein-protein InteractionsF-actin

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