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Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis
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1Lester and Sue Smith Breast Center,Baylor College of Medicine, 2Department of Molecular and Cellular Biology,Baylor College of Medicine, 3Graduate Program in Developmental Biology,Baylor College of Medicine, 4Department of Molecular and Human Genetics,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine, 6Dan L. Duncan Cancer Center,Baylor College of Medicine
Capítulos
- 00:05Título
- 01:16Common Iliac Vein and Artery Location and Separation
- 03:33Injection and Post-injection Care
- 04:45Results: Representative Post Intra-iliac Artery Injected Tumor Cell Localization
- 05:54Conclusion
This manuscript provides the detailed procedure of intra-iliac artery (IIA) injection, a technique to deliver cancer cells specifically to hind limb tissues including bones to establish experimental bone metastases. Although initially established with breast tumor models, this protocol can be easily extended to other cancer types.
