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高内涵筛选分析法评价有害和潜在有害成分的毒性效应(HPHC)
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
DOI:

11:38 min

May 10, 2016

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Capítulos

  • 00:05Título
  • 01:03Real Time Cell Analyzer (RTCA)-based Dose Range Finding (DRF)
  • 04:26Preparation for Measuring Toxicological Effects by High-content Screening (HCS)
  • 06:27Cytotoxicity Assay
  • 09:19Results: Representative Results for 1-Aminonaphtalene
  • 10:26Conclusion

Summary

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The objective of the study was to assess the biological impact of 15 cigarette smoke constituents using a combination of an impedance-based real time cell analyzer and a high-content screening (HCS)-based platform for toxicological assessment in vitro. This study provides information on effective doses, toxicity and modes of action of the tested compounds.

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