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Análise alto teor de triagem para avaliar os efeitos toxicológicos de Constituintes nocivas e potencialmente prejudiciais (HPHC)
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
DOI:

11:38 min

May 10, 2016

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Capítulos

  • 00:05Título
  • 01:03Real Time Cell Analyzer (RTCA)-based Dose Range Finding (DRF)
  • 04:26Preparation for Measuring Toxicological Effects by High-content Screening (HCS)
  • 06:27Cytotoxicity Assay
  • 09:19Results: Representative Results for 1-Aminonaphtalene
  • 10:26Conclusion

Summary

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The objective of the study was to assess the biological impact of 15 cigarette smoke constituents using a combination of an impedance-based real time cell analyzer and a high-content screening (HCS)-based platform for toxicological assessment in vitro. This study provides information on effective doses, toxicity and modes of action of the tested compounds.

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