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Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

JoVE Journal
Medicina

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JoVE Journal Medicina

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

DOI:

10.3791/53994-v

•

06:00 min

•

May 14, 2016

•

Russell T. Burke, James D. Orth

¹Department of Molecular, Cellular, and Developmental Biology,University of Colorado, Boulder

Capítulos

00:05Título
00:22Preparation of Samples and Environmental Chamber Set-up
02:01Microscope and Imaging Set-up
04:03Results: Time Lapse Tracking of Single Cells to Study Anti-cancer Therapeutics
05:35Conclusion

Summary

Tadução automática

Here, we describe a method of long-term time-lapse microscopy to longitudinally track single cells in response to anti-cancer therapeutics.

Tags

Keywords: Time-lapse Microscopy Longitudinal Tracking Single Cells Anti-cancer Therapeutics Cell Culture HT1080 Cells Environmental Chamber Temperature Control Humidity Control Microscopy Setup Imaging Conditions Photo Toxicity Wavelengths Time-lapse Imaging

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