/

/

在新鲜分离的人代绒毛滋养细胞转染和EMSA分析

JoVE Journal
Genética

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Genética

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Please note that all translations are automatically generated. Click here for the English version.

在新鲜分离的人代绒毛滋养细胞转染和EMSA分析

DOI:

10.3791/53995-v

•

09:57 min

•

September 20, 2016

•

Adjimon Gatien Lokossou, Chirine Toufaily, Amandine Vargas, Benoit Barbeau

¹Department of Biological Sciences,University of Quebec in Montreal, ²Department of Pharmacology and Therapeutics,McGill University, ³Department of Clinical Sciences,University of Montreal

Capítulos

00:05Título
01:00Evaluation of the Purity of Isolated Primary Villous Cytotrophoblasts
03:13siRNA Transfection of Human Primary Villous Cytotrophoblasts
04:55Preparation of Nuclear Extracts from Cultured Cells
05:52Radiolabeling of Single Stranded Oligonucleotide
06:50DNA Binding Reaction
08:07Results: Syncytin-2 Knowckdown and Visualization of Syncytin-2 DNA-protein Complexes
09:14Conclusion

Summary

Tadução automática

这个协议描述了使用微孔化有效转染的siRNA在新鲜分离的人绒毛细胞滋养细胞和鉴定在这些细胞中DNA的蛋白质复合物的方法。转染的细胞可以通过Western印迹进行监测，并在为期4天的培养时间，EMSA分析。

Tags

Keywords: SiRNA Transfection EMSA Human Villous Cytotrophoblasts Placenta Research Gene Expression Cytotrophoblast Differentiation Regulatory Mechanisms FACS Analysis CK7 Positive Cells Flow Cytometry

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

接合交配测定在接合介入转移蛋白的序列特异性分析

通过诱导瞬时转染可控离子通道表达

激光局部补骨脂素加合物的单分子分析

重组腺相关病毒9型 MicroRNA 系统分娩治疗啮齿动物神经肌肉疾病

CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究

基于细菌选择和生长的盘状细胞基因工程

使用声学纳米分配技术实现高通量DNA质粒多路复用和转染

基于质谱的蛋白质组学分析, 利用 OpenProt 数据库揭开从非规范开放阅读框架翻译的新蛋白质

从小鼠横纹肌瘤分离的原发性肿瘤细胞的肿瘤球衍生和治疗

通过全基因组互惠性分析,对糖母菌菌种间热耐性差异的遗传图谱

Read Article