Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes

Journal

Biologia

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes

DOI:

10.3791/54012-v

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08:42 min

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August 28, 2016

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Justin Judd, Jonathan Lovas, Guo N. Huang

¹Cardiovascular Research Institute,University of California, San Francisco

Capítulos

00:05Título
00:56Heart Extraction and Cannulation
04:14Mechanical Heart Dissociation and Purification
06:51Results: Representative Isolated Mouse Cardiomyocytes
07:46Conclusion

Summary

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This protocol describes a step-by-step method for the reproducible isolation and long-term culture of adult mouse cardiomyocytes with high yield, purity, and viability.

Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes

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Capítulos

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