/
/
Die Erzeugung von ESC-derived Maus Airway epithelialen Zellen unter Verwendung von Dezellularisierte Lung Scaffolds
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:47Scaffold Preparation
- 03:57Thick Section Generation
- 07:17Decontamination of Scaffold Sections and Recellularization Setup
- 09:46Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
- 11:52Conclusion
Dieses Protokoll leitet effizient embryonalen Maus-Stammzellen abgeleiteten endgültigen Endoderm Epithelzellen der Atemwege zu reifen. Diese Differenzierung Technik verwendet 3-dimensional dezellularisierte Lungengerüste Lungen lineage Spezifikation, in einem definierten, serumfreien Kultureinstellung zu lenken.
Tags
Decellularized Lung ScaffoldsEmbryonic Stem Cell DifferentiationAirway Epithelial Cells3D CultureLung DevelopmentCell Matrix ContractionsGrowth Factor SignalingSerum-free SettingRobust Airway Epithelial DifferentiationLimited Endoderm Lineage ContaminationRat LungsPerfusionTracheal CannulationDecellularization Solution
