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Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster
JoVE Journal
Biologia
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JoVE Journal Biologia
Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster

Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster

DOI:
10.3791/54045-v

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10:01 min

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June 27, 2016

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1Department of Pharmacology,University of Virginia

Capítulos

  • 00:05Título
  • 00:48Preparation of Fly Food Containing Radiolabeled Metabolic Substrates
  • 02:16Feeding Radiolabeled Metabolic Substrates to Adult Fruit Flies
  • 04:03Preparation of the CO2-Collection Apparatus
  • 06:51Analysis of Results
  • 07:45Resultados
  • 09:05Conclusion

Summary

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This paper describes a method for the measurement of fuel oxidation in Drosophila melanogaster in which trace amounts of specific radiolabeled metabolic substrates are fed to flies. The exhaled radiolabeled CO2 that is a produced from fuel oxidation is collected and measured.

Tags

Keywords: Carbon Dioxide ProductionRadiolabeled SubstratesDrosophila MelanogasterEnergy MetabolismFuel OxidationCarbon-14Fly Food VialCarbon Dioxide AnesthesiaFood Dye

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