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Аденовируса генной терапии для больных сахарным диабетом Кератопатия: Влияние на заживление ран и стволовых клеток маркера выражение в человеческих органов культивируемых роговиц и лимбальных эпителиальных клеток
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
DOI:

11:13 min

April 07, 2016

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Capítulos

  • 00:05Título
  • 01:09Epithelial Wound Healing in Organ-cultured Corneas
  • 02:57Adenoiral Transduction of Organ-cultured Corneas
  • 04:31Isolation of Limbal Cells and Maintenance of Stem Cell Enriched Cultures
  • 06:28Adenoiral Transduction of Cultured Limbal Cells
  • 07:55Results: Effects of Gene Therapy on Wound Healing and Stem Cell Marker Expression
  • 10:25Conclusion

Summary

Tadução automática

An example of adenoviral gene therapy in the human diabetic organ-cultured corneas is presented towards the normalization of delayed wound healing and markedly reduced epithelial stem cell marker expression in these corneas. It also describes the optimization of this process in stem cell-enriched limbal epithelial cultures.

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