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سرعة التحقق من صحة ترمينتورس استخدام الموارد الوراثية النباتية الأزرق البلازميد والجمعية جولدن جيت
JoVE Journal
Biologia
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JoVE Journal Biologia
Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
DOI:

09:51 min

April 25, 2016

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Capítulos

  • 00:05Título
  • 01:28Annealing Oligonucleotides (Applies Only to Freeze-dried DNA)
  • 02:43Golden Gate Assembly (Cost Efficient Method Using a Standard Mix)
  • 04:19Transformation and Colony Selection
  • 05:19Verification and Quantification of Terminator
  • 07:55Quantification of Six Different Terminators in pGR-Blue
  • 09:02Conclusion

Summary

Tadução automática

This protocol utilizes Golden Gate Assembly and the plasmid pGR-blue to rapidly quantify the strength of terminators found in silico.

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