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Cryo-tarama elektron mikroskobu tarafından Freeze-kırık Yaprak Dokular içinde Fotosentez Membranlarının Supramoleküler organizasyonu incelenmesi
JoVE Journal
Biologia
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JoVE Journal Biologia
Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy
DOI:

13:52 min

June 23, 2016

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Capítulos

  • 00:05Título
  • 01:12Cryo-fixation of Leaf Tissues by High-pressure Freezing
  • 03:43Freeze Fracture and Double-layer Coating
  • 07:10Cryo-scanning Electron Microscopy
  • 10:52Results: Cryo-scanning Electron Microscopy Analysis of Photosynthetic Membranes in Freeze-fractured Leaf Tissues
  • 12:57Conclusion

Summary

Tadução automática

Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.

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