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Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
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JoVE Journal Medicina
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

DOI:
10.3791/54078-v

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09:39 min

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July 29, 2016

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, , , , ,
1Department of Biology and Biochemistry,University of Houston, 2Department of Economics,University of Houston, 3Department of Cardiology,The First Affiliated Hospital of Nanchang University

Capítulos

  • 00:05Título
  • 00:40C2C12 Transfection
  • 02:06C2C12 Retroviral Transduction
  • 04:44C2C12 Lentiviral Transduction
  • 06:46Results: The Effects of Celf1 in Myoblast Differentiation
  • 08:53Conclusion

Summary

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In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation.

Tags

Keywords: C2C12 MyoblastsMyotonic DystrophyM1 Cell ModelGene ManipulationTransfectionTransductionRetrovirusPackaging CellsSelection AntibioticCell CultureCell MorphologyCytotoxicity

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