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Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

DOI:
10.3791/54097-v

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08:07 min

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June 17, 2016

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, ,
1Department of Biomedical Engineering,National University of Singapore, 2Singapore Institute of Neurotechnology (SINAPSE),National University of Singapore

Capítulos

  • 00:05Título
  • 01:02Preparing the Stencil for Cell Seeding
  • 02:32Seeding Human Embryonic Stem Cells onto the Stenciled Substrate
  • 04:39Stencil Removal and Passivation of Unpatterned Substrate
  • 06:20Results: Mechanically Patterning Early Mesoendermal Differentiation
  • 07:39Conclusion

Summary

Tadução automática

Tadução automática

Human pluripotent stem cells (hPSCs) have the intrinsic ability to differentiate and self-organize into distinct tissue patterns; although this requires the presentation of spatial environmental gradients. We present stencil micropatterning as a simple and robust method to generate biochemical and mechanical gradients for controlling hPSC differentiation patterns.

Tags

Keywords: Stencil MicropatterningHuman Pluripotent Stem CellsSpatial OrganizationStem Cell DifferentiationCell-cell AdhesionCell-matrix AdhesionPDMS StencilBasement Membrane MatrixPlasma TreatmentHESC Colonies

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