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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

DOI:

10.3791/54102-v

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07:37 min

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June 26, 2016

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Manuela Ferracin, Irene Salamon, Laura Lupini, Elena Miotto, Silvia Sabbioni, Massimo Negrini

¹Department of Experimental, Diagnostic and Specialty Medicine – DIMES,University of Bologna, ²Department of Morphology, Surgery and Experimental Medicine,University of Ferrara, ³Department of Life Sciences and Biotechnology,University of Ferrara, ⁴Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA),University of Ferrara

Capítulos

00:05Título
01:21Droplet Generation and PCR
03:50Droplet Reading
05:29Absolute Quantification of microRNA in Plasma Samples
06:50Conclusion

Summary

Tadução automática

A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.

Tags

Keywords: Circulating MicroRNA Droplet Digital PCR DNA-binding Dye Chemistry Biomarker Cancer Diagnosis Plasma Serum Reverse Transcription DdPCR Mix CDNA Template Droplet Generation

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